Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this N-hydroxysuccinimide modification reagent.

Optimize applications where product integrity and risk minimization are paramount with the specially characterized Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS, our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent.

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (–COOH) for conjugation with primary amines (–NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Highlights:

• Sulfo-NHS – use with EDC crosslinker to prepare amine-reactive NHS ester reagents
• High quality – identity and purity confirmed by several tests, including quantitative NMR
• Product integrity – enhanced level of testing and characterization compared to standard grade
• Lot retention – ample supply of past lots retained to ensure future process testing
• Change management – Change Control Notification (CCN) service
• Consistent manufacture – batch-specific manufacturing documentation review

Applications:

• Improve efficiency of EDC coupling reactions
• Convert carboxyls to amine-reactive sulfo-NHS esters
• Membrane-impermable for cell surface labeling
• Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• Activate nanoparticles with amine-reactive sulfo-NHS esters

Chemical Reactivity

Amine-Carboxyl

Cleavable

No

Molecular Weight (g/mol)

217.13

PEGylated

No

Content And Storage

Upon receipt store at 4°C.

Cell Permeability

No

Shipping Condition

Ambient

Product Line

Pierce™

Labeling Method

Chemical Labeling

Crosslinker Type

Heterobifunctional

Reactive Moiety

Carbodiimide

Spacer

Short (<10 Å)

Form

Powder

Quantity

10 g

Solubility

Water

Format

Standard, Single-use, Premium-grade

Water Soluble

Yes

Product Type

Crosslinker

For Research Use Only. Not for use in diagnostic procedures.