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Thermo Scientific™ Pierce™ Fluorescent Protease Assay Kit
Shop All Thermo Scientific ProductsThermo Scientific™ Pierce Protease Assay Kits measure total protease activity in samples, providing a means of assessing the progress of protease isolation procedures or quantifying protease contamination in biological samples.
Highlights:
Casein substrate – measure activity of any protease that cleaves casein into peptide fragments
Trypsin standard – quantify protease activity relative to trypsin, a universally accepted reference
Sensitive – 1000 times more sensitive than assays that use unmodified forms of casein
Simple and fast – test tube and microplate protocols are completed in less than one hour
Homogenous – addition steps only; no separation, transfer or stop steps required
Customizable – easily adapt time, temperature and pH to optimize sensitivity for proteases of interest
- Substrate: succinylated casein (not available separately)
- Basis of assay: detection of primary amines resulting from proteolysis
- Detection reagent: trinitrobenzene sulfonic acid (TNBSA)
- Measurement: absorbance at 450nm (plate reader or spectrophotometer)
- Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267
- Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP)
- Measurement: instrument with fluorescein excitation and emission filters (485/538nm)
Includes:
Modified casein, trypsin standard and Tris-buffered saline (both kits); TNBSA solution (Part No. 23263 only)
Recommended for:
Assess functional integrity and overall progress of protease purification; Quantify protease contamination in protein samples; Characterize the activity of unusual proteases compared to trypsin; Evaluate buffer conditions for their affects on protease function and activity
Specifications
Content And Storage
Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm)
• FTC-Casein, lyophilized, 2.5 mg
• TPCK Trypsin Standard, 50 mg
• TBS Buffer Pack (makes 500 mL), 1 pack
Store FTC-Casein and TPCK Trypsin at 4°C.
Assay
Protease Assay
Detection Method
Fluorescence
Immunoassay Kit Format
96-well Plate
Label Type
Classic Dye
Product Line
Pierce™
Product Type
Protein Activity Assay
Substrate
Casein
Substrate Properties
Protein-Based Substrate
Substrate Type
Protease Substrate
For Research Use Only. Not for use in diagnostic procedures.
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