Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Lipid)

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.

The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (lipid-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This lipid-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit's formulation may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

Features of the Quantitative Peroxide Assay Kit:
• Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
• Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself

Applications:
• General hydrogen peroxide (H₂O₂) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this lipid-compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560 nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Related Products
Pierce™ Quantitative Peroxide Assay Kit, Aqueous (Cat. No. 23280)
Pierce™ Peroxide Assay Reagent A (Cat. No. 23281)
Pierce™ Peroxide Assay Reagent B (Cat. No. 23282)
Pierce™ Peroxide Assay Reagent C (Cat. No. 23283)

Content And Storage

Sufficient For: 100 tube assays or 500 microplate assays with lipid-containing samples
• Reagent A, 1 mL
• Reagent C, 4 x 25 mL

Store at 4°C.

Description

Pierce Quantitative Peroxide Assay Kit (Lipid)

Assay

Quantitative Peroxide Assay Kit

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Quantitative Peroxide Assay

Specificity

Not Target-Specific

Sufficient For

100 Tube Assays or 500 Microplate Assays

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

For Research Use Only. Not for use in diagnostic procedures.