Thermo Scientific™ Rapid ELISA Mouse mAb Isotyping Kit

Isotype mouse monoclonal antibody using an antigen-independent, microplate-based method that enables multiple samples to be tested in about one hour.

Thermo Scientific™ Pierce Rapid ELISA Mouse Antibody Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.

The kit enables easy identification of mouse immunoglobulin class, subclass and light chain composition. This fast assay uses ELISA strip-plates pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype. A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.

Highlights:

• Fast – determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits

• Convenient – eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)

• Specific – characterize antibodies for six different subclasses and two different light-chain types

• Sensitive – accurate characterization with samples containing more than 3ng/mL of test antibody

• Flexible – kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies

• Cost effective – cost per assay is less than with single-use assay strips or cassettes

• No special equipment needed – assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)

• Complete and easy to use – includes pre-coated plates, detecting antibody, buffers, and TMB substrate and stop solutions

Includes:

Precoated 96-well Isotyping plates, 5 plates; Goat anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30mL; TMB Substrate, 55mL; Stop solution, 55mL; 30X Wash buffer, 200mL; Tris buffered saline, 2 packs

Target Specificity

Target-specific

Product Line

Pierce™

For Use With (Application)

ELISA (Antigen Capture), Absorbance

Quantity

60 Tests

Content And Storage

Sufficient For: 60 isotype determinations (8 wells per test sample)
• Pre-coated 96-well Isotyping Plates, 5 plates
• Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL
• TMB Substrate, 55 mL
• Stop Solution, 55 mL
• 30X Wash Buffer, 200 mL
• TBS Buffer Packs (each makes 500 mL), 2 packs

Store at 4°C.

For Use With (Equipment)

Microplate Reader

Assay

Isotyping Assay

Detection Method

Colorimetric

For Research Use Only. Not for use in diagnostic procedures.