Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based Renilla luciferase kit.

The Thermo Scientific™ Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.

This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates. When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression.

Highlights:

• Sensitive – greater sensitivity allows utilization of smaller numbers of cells

• Cost effective – highest sensitivity assays result in decreased reagent consumption

• Time-saving – assays require minimal sample handling

• Automation-friendly – amenable to high throughput screens

• Convenient – contains a universal cell lysis buffer and optimized flash assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Type

Assay Kit

Target

Luciferase, Green Renilla Luciferase

Quantity

1000 Reaction Kit

Content And Storage

• Renilla Flash Assay Buffer, 50 mL, store at 4°C
• Coelenterazine (100X), 0.5 mL, store at -80°C
• 2X Cell Lysis Buffer, 60 mL, store at room temperature

Assay

Reporter Enzyme, Luciferase Reporter Assay

Compatible Cells

Mammalian Cells

Detection Method

Bioluminescence

For Use With (Equipment)

Luminometer (Microplate)

Format

384-well plate, 96-well plate

Label Type

Enzyme Labeled

Substrate Properties

Chemical Substrate

Technique

Enhanced Chemiluminescence

Substrate Type

Luciferase Substrate

Product Line

Pierce™

Substrate

Coelenterazine

For Research Use Only. Not for use in diagnostic procedures.