Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.

Thermo Scientific™ Pierce Renilla Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of Green Renilla luciferase reporter.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of Renilla luciferase in cell lysates. In the presence of luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Green Renilla Luc Plasmids; however, this glow kit may be used to detect the activity of other Renilla luciferase derivatives that use coelenterazine as a substrate.

Highlights:

• Sensitive – highly sensitive detection of Green Renilla luciferase activity

• Stable – increased signal stability compared to flash assays

• Simple – does not require a luminometer with injectors

• Compatible – assay reagents compatible with other Renilla luciferases

• Automation-friendly – amenable to high throughput assays

• Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan™ Ascent and Varioskan™ Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Type

Assay Kit

Target

Luciferase, Green Renilla Luciferase

Quantity

1000 Reaction Kit

Content And Storage

Upon receipt store kit at -80°C or store individual components as indicated above.
• Renilla Glow Assay Buffer, 50 mL, store at 4°C
• Coelenterazine (100X), 0.5 mL, store at -80°C
• 2X Cell Lysis Buffer, 60 mL, store at room temperature

Assay

Reporter Enzyme, Luciferase Reporter Assay

Compatible Cells

Mammalian Cells

Detection Method

Bioluminescence

For Use With (Equipment)

Luminometer (Microplate)

Format

384-well plate, 96-well plate

Label Type

Enzyme Labeled

Substrate Properties

Chemical Substrate

Technique

Enhanced Chemiluminescence

Substrate Type

Luciferase Substrate

Product Line

Pierce™

Substrate

Coelenterazine

For Research Use Only. Not for use in diagnostic procedures.