Thermo Scientific™ Powdered DMEM Medium for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

These Thermo Scientific Pierce cell culture media and serum components are optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

• Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

• Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

• High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation

Formulation

DMEM media minus L-Leucine, L-Lysine and L-Arginine

For Use With (Equipment)

Mass Spectrometer

Product Type

Media

Content And Storage

135 g of powdered medium to make 10 L of medium.

Store in refrigerator (2 to 8°C).

Shipping Condition

Wet Ice

Starting Material

Cells

Final Product Type

Protein

Workflow Step

Protein Labeling

Quantity

135 g

Form

Powder

For Research Use Only. Not for use in diagnostic procedures.