Thermo Scientific™ Pierce™ Thiophilic Adsorption Kit

Description

Preferentially bind and purify immunoglobulins from various sources including mouse, rat, rabbit, goat and human with this non-proteinaceous immunosorbent resin.

Thermo Scientific™ Pierce Thiophilic Adsorbent is a non-proteinaceous immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human.

Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity. The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers.

Highlights:

• High capacity – gently bind and purify approx. 20mg polyclonal antibody per milliliter of adsorbent resin

• Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY

• Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps

• Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody

Includes:

Kit contains four Thiophilic Adsorbent Columns (3mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories

Recommended for:

Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications

Specifications

• Product Type: Adsorption Kit
• Product Line: Pierce™
• Column Type: Agarose Resin, Affinity
• Format: Gravity Flow Column
• Stationary Phase: Sulfone Thioether
• Purification Target: IgG Antibody
• Description: Pierce Thiophilic Adsorption Kit
• Content And Storage: Sufficient For: Purifying up to 60 mg immunoglobulin per column
  • Thiophilic Adsorbent Columns, 3 mL, 4 columns
  • Binding Buffer, 1 L
  • Elution Buffer, 1 L
  • Column Storage Buffer (2X), 100 mL
  • Guanidine-HCl Crystals, 230 g
  • Column Accessories
  
  Store at 4°C.
• Quantity: 4 Columns

For Research Use Only. Not for use in diagnostic procedures.