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Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis
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Thermo Scientific™ Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.
Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250 U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.
Highlights:
- Broad spectrum – degrades all forms of DNA and RNA
- Highest-quality enzyme – nuclease is at least 99% pure, as tested by SDS-PAGE
- Robust activity – 100-fold greater specific activity than DNase I
- Versatile – can be used with a wide variety of cell lysis reagents
Recommended for:
Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing
Storage Requirements
Store in a cool, dry, well-ventilated area, protected from direct sunlight
Specifications
Specifications
| Formulation | At least 99% pure enzyme at 250 U/uL in 50% glycerol (v/v), 20 mM NaCl, 2 mM MgCl2, 20 mM Tris, pH 8.0 |
| Description | Pierce Universal Nuclease for Cell Lysis |
| Product Type | Cell Lysis Enzyme |
| Content And Storage | Store in a cool, dry, well-ventilated area, protected from direct sunlight. |
| Form | Liquid |
| Product Line | Pierce™ |
| Reagent Type | Enzyme for Cell Lysis |
| Enzyme | Nuclease |
| Sufficient For | 200 mL Lysate |
| Quantity | 5 kU |
For Research Use Only. Not for use in diagnostic procedures.
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