Thermo Scientific™ Yeast DNA Extraction Kit

Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.

Quickly and easily lyse yeast cells to release DNA for study with Thermo Scientific™ Pierce Yeast DNA Extraction Kit, which utilizes a Thermo Scientific γ-PER Reagent.

The kit provides greater yields than traditional methods of DNA isolation from yeast. Typically, extraction and purification of DNA from yeast are time-consuming and labor-intensive. The yeast proteinaceous cell wall is notoriously difficult to lyse and requires harsh treatments that are time-consuming and can damage the extracted DNA. The Yeast DNA Extraction Kit protocol requires less than one hour to complete, is effective without enzymatic treatment or glass beads, and yields little to no RNA contamination regardless of RNase presence. In studies with Saccharomyces cerevisiae, high yields of genomic and plasmid DNA are consistently obtained. DNA purified using this kit is suitable for PCR amplification, bacterial transformations, restriction digestions and hybridization applications.

Highlights:

• Eliminates need for glass beads or harsh enzyme treatments
• Yields DNA that is compatible with polymerase chain reaction (PCR) amplification
• Rapidly isolates plasmid DNA from S. cerevisiae suitable for transformation of E. coli
• Protocol is easily scalable for use with single colonies or 500mL cultures

Includes:

Thermo Scientific γ-PER Yeast Protein Extraction Reagent, DNA releasing reagents A and B, and protein removal reagent

Content And Storage

Sufficient For: 50 purifications from 10 mL cultures
• Y-PER Yeast Protein Extraction Reagent, 25 mL
• DNA Releasing Agent A, 20 mL
• DNA Releasing Agent B, 20 mL
• Protein Removal Reagent, 10 mL

Store at 4°C. Precipitates may form; gently warm at 37°C for 1–5 minutes.

Sample Type

Yeast

For Use With (Application)

PCR, Sequencing, Transformation

High-throughput Compatibility

Not High-throughput Compatible

Product Type

Yeast DNA Extraction Kit

Quantity

75 mL

Target

Genomic DNA

For Research Use Only. Not for use in diagnostic procedures.