Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Supplier:  Invitrogen™ 13000014

Catalog No. 13-000-014

Description

Invitrogen Platinum Hot Start PCR Master Mix, available in colorless and green formats, offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. It retains all the features of Platinum Taq DNA Polymerase: specificity, robustness, and reliability.

Platinum Green Hot Start PCR Master Mix additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels.

Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets.

Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step, and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.

Features

• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Versatile formulation for broad range of amplicons
• Direct gel loading with green PCR master mix

Applications

• Hot Start PCR
• Genotyping
• Gene expression profiling
• Next-generation sequencing

Notes

• Platinum II Taq Hot-start DNA Polymerase is recommended superior PCR performance.
• For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.

Specifications

• Content And Storage: • Platinum Hot Start PCR 2X Master Mix, 2 x 12.5 mL
  • Platinum GC Enhancer, 10 mL
  • Nuclease-free water, 2 x 12.5 mL
  
  Store at -20°C in a non-frost-free freezer.
• GC-Rich PCR Performance: Medium
• Reaction Speed: Standard
• Product Type: Hot Start PCR Master Mix
• Shipping Condition: Dry Ice
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less
• Color: Colorless

• Format: Tube
• Polymerase: Platinum Taq DNA Polymerase
• Label or Dye: Unlabeled
• Quantity: 1000 reactions
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 1000 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.