Mirus Bio™ pOET1C_6xHis Transfer Plasmid

pOET1C_6xHis Transfer Plasmid for high-yield protein production in insect cells.

Baculovirus transfer plasmid designed for use with the flashBAC™ Baculovirus Expression System and TransIT™-Insect Transfection Reagent. The powerful AcMNPV polyhedrin promoter affords high-level gene expression in insect cells. A C-terminal 6xHis-tag fusion sequence is included for recombinant protein purification with Ni-NTA agarose columns and subsequent removal by thrombin cleavage.

• Simple construction of recombinant virus when used with the flashBAC™ Baculovirus Expression System
• Can be used with any baculovirus expression system that relies on recombination in insect cells at the polyhedrin locus
• Can be propagated in DH5α or other general purpose E. coli strains

Vector Features:

• Col E1 origin of replication
• Ampicillin resistance gene for selection in E. coli
• Multiple cloning site for insertion of the foreign gene in the correct frame and orientation
• C-terminal 6xHis-Tag™ fusion sequence

Warranty and Services

1 Year

Concentration

500 ng/μL, Sterile filtered in TE Buffer

Content And Storage

Store at -20°C

Format

Kit

For Use With (Application)

Protein production in insect cells

Quantity

20 μL