Mirus Bio™ pOET6 BacMam Transfer Plasmid

pOET6 BacMam Transfer Plasmid for high-yield protein production in mammalian cells.

Baculovirus transfer plasmid designed for use with the flashBAC™ Baculovirus Expression System and TransIT™-Insect Transfection Reagent. The CMV promoter enables high-level gene expression in mammalian cells.

• Simple construction of recombinant virus when used with the flashBAC™ Baculovirus Expression System
• Can be used with any baculovirus expression system that relies on recombination at the polyhedrin locus
• Can be propagated in DH5α or other general purpose E. coli strains

Vector Features:

• Col E1 origin of replication
• Ampicillin resistance gene for selection in E. coli
• CMV promoter to drive constitutive foreign gene expression
• Multiple cloning site containing 11 unique restriction sites for inserting the gene in the correct orientation

Warranty and Services

1 Year

Concentration

500 ng/μL, Sterile filtered in TE Buffer

Content And Storage

Store at -20°C

Format

Kit

For Use With (Application)

Protein production in mammalian cells

Quantity

20 μL