Thermo Scientific™ Poly(A) Polymerase, Yeast

Catalyses the addition of adenosine residues onto the 3' ends of RNA. It can be used to add poly(A) tails to RNA in the first step of cloning.

Supplier:  Thermo Scientific™ 74225Z25KU

Catalog No. 74-225-Z25KU

Description

• The reaction requires Mn²⁺ or Mg²⁺, ATP as substrate, and any RNA containing 3' hydroxyl termini as primer.
• Substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single 3'-dA residue to the ends of the RNA, a useful technique for labeling RNA at the 3' end.
• In comparative studies, yeast poly(A) polymerase works more efficiently than E. coli poly(A) polymerase for RNA oligonucleotide-labeling and poly(A) tailing.
• Poly(A) Polymerase is recommended over T4 RNA Ligase for 3'-end labeling of long RNA molecules.
• Ribonuclease free
• 20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM DTT, 50% glycerol.
• One unit equals 15 pmol/min AMP incorporated into (riboA)15 at 37°C.
• 600 units/μL
• 3' -end labeling of a ribonucleotide with cordycepin-5'-triphosphate.
• Addition of poly(A) tails to RNA.;2.Labeling the 3' ends of RNA.
• E. coli strain containing an overproducing clone of Yeast Poly(A) Polymerase.<br

Specifications

• Content And Storage: Shipped on dry ice. Store at -20°C.
• Polymerase: Poly(A) RNA Polymerase
• Quantity: 25 KU

For Research Use Only. Not for use in diagnostic procedures.