Thermo Scientific™ ProPac™ HIC-10 HPLC Columns

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns.

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns. Developed with multi-functional ligand attachments, these silica-based columns provide exceptional hydrolytic stability under the highly aqueous conditions used in HIC.

High-efficiency Columns for the Separation of Large Biomolecules

• High protein loading capacity for protein purification applications
• Wide range of analytical and preparative applications, including the separation of bovine serum proteins, snake venom proteins, and monoclonal antibodies (MAbs) 
• Based on 5μm ultra-high purity spherical silica gel particles with 300Å pores

Hydrophobic interaction chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

Column Format

Analytical Column

Diameter (Metric)

4.6 mm

Length (Metric)

250 mm

Max. Pressure

3500 psi (240 bar)

Particle Size

5 μm

pH

2.5 to 7.5

Pore Size

300 Å

Surface Area

100 m²/g

Temperature

40°C

Stationary Phase

Amide/Ethyl

Product Line

ProPac

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Column Type

Hydrophobic Interaction

Phase

Hydrophobic Interaction

Material

Stainless Steel

Type

Analytical Column

Technique

Bio-LC

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