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  6. Thermo Scientific™ ProPac™ HIC-10 HPLC Columns

Thermo Scientific™ ProPac™ HIC-10 HPLC Columns

Catalog No. p-4523244 Shop All Thermo Scientific Products
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Length (Metric):
100 mm
250 mm
Diameter (Metric):
2.1 mm
4.6 mm

Description

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns.

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns. Developed with multi-functional ligand attachments, these silica-based columns provide exceptional hydrolytic stability under the highly aqueous conditions used in HIC.

High-efficiency Columns for the Separation of Large Biomolecules
  • High protein loading capacity for protein purification applications
  • Wide range of analytical and preparative applications, including the separation of bovine serum proteins, snake venom proteins, and monoclonal antibodies (MAbs) 
  • Based on 5μm ultra-high purity spherical silica gel particles with 300Å pores
Hydrophobic interaction chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

Specifications

Column Format Analytical Column
Diameter (Metric) 2.1 mm
Length (Metric) 100 mm
Max. Pressure 3500 psi (240 bar)
Particle Size 5 μm
pH 2.5 to 7.5
Pore Size 300 Å
Surface Area 100 m²/g
Temperature 40°C
Stationary Phase Amide/Ethyl
Product Line ProPac
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Hydrophobic Interaction
Phase Hydrophobic Interaction
Material Stainless Steel
Type Analytical Column
Technique Bio-LC
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