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  6. Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns

Thermo Scientific™ ProPac™ IMAC-10 HPLC Columns

Catalog No. p-4523591 Shop All Thermo Scientific Products
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Length (Metric):
50 mm
250 mm
Diameter (Metric):
4 mm
9 mm
22 mm
Column Format:
Analytical Column
Loading Column
Particle Size:
10 μm

Description

Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.

Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.

Tailored Specificity for IMAC Separation of Proteins
  • Retention control by imidazole concentration or pH gradient
  • Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
  • Effective under native or denaturing HPLC conditions
  • Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
  • Shipped free from any metal and ready to be charged with the metal of your choice.
  • Reusable columns with metal tailored specificity
  • Resolve target proteins using a single column in a high resolution gradient run
  • High purity separations of metal-binding proteins
Includes: 10μm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.

Specifications

Column Format Analytical Column
Diameter (Metric) 4 mm
Flow Rate 1 mL/min
For Use With UltiMate 3000 HPLC System
Length (Metric) 50 mm
Max. Pressure 3000 psi (207 bar)
Mobile Phase Compatibility pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size 10 μm
pH 2 to 12
Pore Size Nonporous
Temperature 60°C
Stationary Phase Lysozyne Poly(IDA) Grafts
Product Line ProPac
Packing Material Spherical, Nonporous Polymer
Column Type Separation of Metal-Binding Proteins
Phase Affinity Separation
Type Analytical Column
Technique Bio-LC
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