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  6. Thermo Scientific™ ProPac™ SAX-10 HPLC Columns

Thermo Scientific™ ProPac™ SAX-10 HPLC Columns

Catalog No. p-4524075 Shop All Thermo Scientific Products
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Length (Metric):
50 mm
250 mm
Diameter (Metric):
2 mm
4 mm
22 mm
Column Format:
3 Lots of Resin, 1 Column/Lot (3 Columns)
Analytical Column
Guard Column
Semi-Preparative Column
Particle Size:
10 μm

Description

Use ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants.

Use Thermo Scientific™ ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants. Based on a nonporous 10μm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Maximizing Resolution
  • ProPac SAX-10 LC column is a strong anion-exchanger with a quaternary amine functional group.
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.

Superior Performance for Protein Analysis
  • Ideal for characterization and quality control assessment of closely-related protein variants
  • Unequaled high-resolution separations
  • High-efficiency peaks with excellent recovery
  • Higher capacity than other columns based on nonporous particles
  • Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing

Specifications

Column Format Analytical Column
Diameter (Metric) 4 mm
Flow Rate 1 mL/min
For Use With LC
Length (Metric) 250 mm
Max. Pressure 3000 psi (207 bar)
Mobile Phase Compatibility pH 2 to 12, 80% Compatible with Acetonitrile, Acetone, Methanol, Compatible with Nonionic and Zwitterionic Detergents, Incompatible with Anionic Detergents
Particle Size 10 μm
pH 2 to 12
Pore Size Nonporous
Temperature 60°C
USP Type N/A
Stationary Phase Quaternary Ammonium
Product Line ProPac
Packing Material Spherical, Nonporous Polymer
Column Type Ion Exchange
Phase Ion Exchange
Technique Bio-LC
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