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Thermo Scientific™ ProPac™ WAX-10 HPLC Columns

Catalog No. p-4524072 Shop All Thermo Scientific Products
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Length (Metric):
50 mm
250 mm
Diameter (Metric):
2 mm
4 mm
9 mm
22 mm
Column Format:
Analytical Column
Guard Column
Semi-Preparative Column
Particle Size:
10 μm

For exceptionally high resolution and efficiency when separating protein variants, use ProPac WAX-10 LC Columns.

For exceptionally high resolution and efficiency anion-exchange separation of protein variants, use Thermo Scientific™ ProPac™ WAX-10 LC Columns. Based a nonporous 10μm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Maximizing Resolution
  • ProPac WAX-10 LC column is a weak anion-exchanger with a tertiary amine functional group.
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.
Superior Performance for protein analysis
  • Ideal for characterization and quality control assessment of closely-related protein variants
  • Unequaled high-resolution separations
  • High-efficiency peaks with excellent recovery
  • Higher capacity than other columns based on nonporous particles
  • Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing

Specifications

Column Format Analytical Column
Diameter (Metric) 4 mm
Flow Rate 1 mL/min
Length (Metric) 250 mm
Max. Pressure 3000 psi (207 bar)
Mobile Phase Compatibility pH 2 to 12, 80% compatible with acetonitrile, acetone, methanol, compatible with nonionic and zwitterionic detergents, incompatible with anionic detergents
Particle Size 10 μm
pH 2 to 12
Pore Size Nonporous
Temperature 60°C
Stationary Phase Tertiary Amine
Product Line ProPac
Packing Material Spherical, Nonporous Polymer
Column Type Ion Exchange
Phase Ion Exchange
Type Analytical Column
Technique Bio-LC
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