Thermo Scientific™ ProPac™ WCX-10 Carboxylate Ion Exchange HPLC Column, 10 μm, 4 mm x 50 mm

Description

Column Format: Analytical Column; Pore Size: Nonporous; Column Phase: Ion Exchange

Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10μm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Multiple Formats for Greater Flexibility

• ProPac WCX-10 column is a weak cation-exchanger with a carboxylate functional group
• Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semi-prep work

Superior Performance for Protein Analysis

• Ideal for characterization and quality control assessment of MAbs and other proteins
• Well-suited for characterization of related protein variants including deamidation and MAb lysine truncation variants
• Unequaled high-resolution separations
• Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing

Specifications

• Column Format: Analytical Column
• Diameter (Metric): 4 mm
• Flow Rate: 1 mL/min
• Length (Metric): 50 mm
• Max. Pressure: 3000 psi (207 bar)
• Mobile Phase Compatibility: pH 2 to 12, 80% compatible with acetonitrile and acetone, compatible with nonionic and zwitterionic detergents, incompatible with cationic detergents
• Particle Size: 10 μm
• pH: 2 to 12
• Pore Size: Nonporous
• Temperature: 60°C
• Stationary Phase: Carboxylate
• Product Line: ProPac
• Packing Material: Spherical, Nonporous Polymer
• Column Type: Ion Exchange
• Phase: Ion Exchange
• Type: Analytical Column
• Technique: Bio-LC