Molecular Probes™ Propidium Iodide - 1.0 mg/mL Solution in Water

Propidium Iodide - 1.0 mg/mL Solution in Water

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Content And Storage

Contains 1 bottle of propidium iodide(1.0 mg/mL solution in water).
Store in refrigerator (2–8°C) and protect from light.

Detection Method

Fluorescence

For Use With (Application)

Viability Assay

For Use With (Equipment)

Fluorescence Microscope, Flow Cytometer

Product Type

Propidium Iodide

Dye Type

propidium iodide

Emission

533⁄617

Form

Solution

Quantity

10 mL

Shipping Condition

Room Temperature

Sub Cellular Localization

Cytoplasm & Cytosol

For Research Use Only. Not for use in diagnostic procedures.