Learn More
Invitrogen™ Propidium Iodide Ready Flow™ Reagent
Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 535/617 nm.
• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh. or pipette
• Rapid staining of dead cells without wash steps
Propidium Iodide Ready Flow Reagent is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.
Propidium iodide is membrane impermeant and therefore excluded from viable cells. It binds to DNA by intercalating between the bases, thereby binding both DNA and RNA. Propidium iodide is commonly excited with the 488 nm laser, but others such as the 532 and 561 nm lasers are also suited for PI excitation.
Specifications
Cell Permeability
Cell-Impermeant
Color
Red
Content And Storage
2 dropper bottles, store (at room temperature)
Detection Method
Fluorescence
Product Type
Fluorescent Stain
Product Line
Ready Flow™
Quantity
120 assays
Reagent Type
Nucleic Acid Stain
Sub Cellular Localization
Nucleus
Excitation/Emission
535/617
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.