Gibco™ PSC Neural Induction Medium

PSC Neural Induction Medium

Gibco™ PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types.

PSC Neural Induction Medium enables:

Rapid induction of NSCs—high efficiency neural induction in only 7 days, without EB formation

Scalable production of NSCs:—generate >20 million NSCs starting from 1 million PSCs

Generation of high quality NSCs—resulting NSCs can be cryopreserved, expanded, and differentiated

Rapid Induction of NSCs
PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.

Scalable Production of NSCs
PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune™-iPS Sendai Reprogramming Kit.

Generation of High Quality NSCs
Neural stem cells generated using PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes.

Content And Storage

• 500 mL Basal medium (store at 2–8°C and protect from light)
• 10 mL Supplement (store at -5 to -20°C and protect from light)

Cell Type

Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs), Human Stem Cells

Product Type

PSC Neural Induction Media

Serum Level

Serum-free

Quantity

500 mL

For Research Use Only. Not for use in diagnostic procedures.