Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

Thermo Scientific™ QuantaRed Enhanced Chemifluorescent HRP Substrate is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence.

QuantaRed Enhanced Chemifluorescent HRP Substrate enables high-yield and sensitive fluorescent and colorimetric detection of peroxidase activity in the ELISA format. QuantaRed Substrate uses a proprietary technology to increase fluorescent yield and sensitivity of the well-documented ADHP (10-acetyl-3,7-dihydroxyphenoxazine) chemifluorescence reaction. ADHP is a non-fluorescent compound that reacts with horseradish peroxidase (HRP) to produce resorufin, a soluble, highly fluorescent reaction product (excitation/emission approx. 570/585nm). The long wavelength emission of the resorufin minimizes interference from the low wavelength (blue and green) autofluorescence, making it an ideal substrate for quantitating target molecules in biological samples. In addition, the colored reactopm product of QuantaRed Substrate can be measured spectrophotmetrically at 570nm.

Highlights:

• Sensitive – low picogram to femtogram detection

• Red-shifted – less interference from autofluorescence generated by biological samples

• Convenient – compatible with existing filter sets, no need to purchase special filter sets

• Complete – easy to use; no buffers to prepare or reagent powders to dissolve

• Flexible – effective stop solution result in stable signal for at least 4 hours

• Versatile – produces strong signal for easy fluorometric or colorimetric measurement

Note:

Equilibrate all reagents to room temperature before use. Temperature can affect the reaction kinetics.

Product Type

ELISA Substrate

Detection Method

Fluorescence

Product Line

QuantaRed™

Substrate Properties

Chemical Substrate

Substrate Type

HRP (Horseradish Peroxidase)

Content And Storage

Sufficient For: 960 wells (10 x 96-well plates) at 100 μL working solution per well
• QuantaRed ADHP Concentrate, 1 mL
• QuantaRed Enhancer Solution, 50 mL
• QuantaRed Stable Peroxide Solution, 50 mL
• QuantaRed Stop Solution, 10 mL

Store at 4°C.

For Research Use Only. Not for use in diagnostic procedures.