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Reproducibly quantify small RNA (⁓20 nucleotides or base pairs), even in the presence of contaminants such as salts, free nucleotides, solvents, detergents, and protein, with the Qubit microRNA Assay Kit. This assay is highly selective for small RNA over rRNA or large mRNA and can quantify small RNA in pure samples at levels as low as 0.5 ng in the assay tube. The assay detects all types of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA.
Features of the Qubit microRNA Assay Kit
• Accurately detects as little as 0.5 ng miRNA in the assay tube
• Core dynamic range of 5–500 ng/mL.
• Accurate results for sample concentrations ranging from 50 ng/mL to 100 µg/mL
The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations ranging from 50 ng/mL–100 µg/mL.
Specifications
Detection Method
Fluorescence
For Use With (Equipment)
Qubit Fluorometer
For Use With (Application)
microRNA Quantitation
No. of Reactions
100 Reactions
Product Line
Qubit
Product Type
microRNA Assay Kit
Shipping Condition
Room Temperature
Excitation/Emission
498/518
For Research Use Only. Not for use in diagnostic procedures.
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