R&D Systems™ MagCellect Mouse CD4+ CD25+ Regulatory T Cell Isolation Kit

Designed to isolate mouse CD4+CD25+ regulatory T cells using a two-step procedure that combines both negative and positive selection techniques

The resulting cell preparation is highly enriched with CD4+CD25+ T cells with a purity of recovered cells ranging between 84-94%
Isolation of CD4+ T cells by negative selection is done in a test tube and is achieved by tagging unwanted cells with the MagCellect Mouse CD4+ T Cell Biotinylated Antibody Cocktail followed by the addition of MagCellect Streptavidin Ferrofluid
Magnetically tagged cells will migrate toward the tube wall on the magnet side (unwanted cell fraction)
Isolation of CD4+CD25+ regulatory T cells from the CD4+ T cell isolated fraction, is done by positive selection in a test tube by tagging the cells of interest with an anti-mouse CD25 biotinylated antibody followed by the addition of the Streptavidin Ferrofluid
Magnetically tagged cells will migrate toward the tube wall on the magnet side (desired cell population), leaving the untagged (unwanted) cells in suspension

Content And Storage

Store the unopened product at 2°C to 8°C. Do not use past expiration date.