Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to protein standard curve of known concentrations.

• A fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration
• Can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications
• Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods
• Provides comparable accuracy but with a 5 min., room temperature incubation and measured at 480 nm
• This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results
• Exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
• Unaffected by typical concentrations of most ionic and non-ionic detergents
• Linear working range for BSA of 20 to 2000 µg/mL
• Store in refrigerator at 2°C to 8°C

Assay Range

20 to 2000 μg/mL

Content And Storage

Store in refrigerator (2–8°C).

Assay

Rapid Gold BCA Assay

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Protein Quantitation Assay

Specificity

Not Target-Specific

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

Quantity

20 mL

Shipping Condition

Wet Ice

For Research Use Only