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Innovative Research Inc Rat Coagulation FX Kit
Rat Coagulation Factor X ELISA Kit - Rat Coagulation Factor X ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Coagulation Factor X in biofluid samples, such as plasma. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . F10 present in samples reacts with an anti-Rat F10 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-F10 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active F10 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat F10. For RUO
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