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Raw 264.7 (mouse macrophage, Abelson murine leukemia virus transformed) Whole cell lysate, Non-denatured; Abnova
Shop All Abnova Corporation ProductsWestern Blotting, Immunoprecipitation
Specifications
Tissue
Macrophage
For Use With (Application)
Immunoprecipitation, Western Blot
Description
Whole cell lysate (non-denatured)
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity
200 μg
Storage Buffer
In modified RIPA Lysis Buffer.
Host Species
Mouse
Content And Storage
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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