Invitrogen™ ReadyProbes™ 2.5% Normal Horse Serum (1X)

Ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments

• ReadyProbes 2.5% Normal Horse Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a horse or donkey secondary antibody is used.
• Incubating cells/tissue with diluted serum from the same or similar species as the host of the secondary antibody typically reduces non-specific binding.
• Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Horse Serum and incubate for 30–60 minutes at room temperature.
• Ready Probes 2.5% Normal Horse Serum can also be used to dilute primary and secondary antibodies.
• Ready Probes 2.5% Normal Horse Serum is provided as a ready-to-use reagent with no further dilution or preparation required.
• The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay.
• The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.

Content And Storage

Provided as 50-mL solution at ready-to-use (1X) concentration in a clear plastic dropper bottle.

Store at 2–8°C.

When stored as directed, product can be used for 6 months after receipt.

Species

Horse

Form

Liquid

Product Type

Horse Serum

Quantity

50 mL

Product Line

ReadyProbes™

Reagent Type

Blocking Reagent

Shipping Condition

Room Temperature or Wet Ice

For Research Use Only. Not for use in diagnostic procedures.