Invitrogen™ ReadyProbes™ Reagent F-Actin Phalloidin Conjugates

Includes

2 x 2.5 mL dropper bottles

Perfect for fixed cells and tissue, ReadyProbes Reagent F-actin phalloidin conjugates offer clear visualization of actin filaments, making it perfect for cell biology, neuroscience, cancer research, and more.

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle.

These ready-to-use solutions combines superior brightness with exceptional F-actin staining. Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

Features

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• ActinGreen 488 Excitation/Emission: (495/518 nm), detected through standard GFP and FITC filter sets
• ActinRed 555 Excitation/Emission: (540/565 nm), detected through standard RFP and TRITC filters sets
• Use counterstain (i.e., DAPI or NucBlue ReadyProbes reagent) as desired

ReadyProbes format advantages

• Ready-to-use formulation—no need to dilute, weigh, or pipette
• Just use two drops per mL of medium to stain, incubate 30 minutes, wash, then image
• Stable at room temperature—keep handy on the benchtop

Applications

• For fixed and permeabilized cells or tissue
• Fluorescence microscopy (IF/IHC/ICC)
• High content screening, high content imaging
• Flow cytometry

Suggestions for use

• Both ActinRed 555 and ActinGreen 488 reagents may be added directly to fixed cells in full medium or buffer solutions
• In most cases 2 drops/mL and an incubation time of 15–30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

Color

Green

Content And Storage

2 x 2.5 mL dropper bottles

Store at ≤ 25°C.

Excitation Wavelength Range

495/518

Dye Type

Alexa Fluor™ 488

For Use With (Equipment)

Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System

Quantity

1 kit

Detection Method

Fluorescence

Description

ActinGreen™ 488 ReadyProbes™ Reagent (AlexaFluor™ 488 phalloidin)

Emission

Visible

Form

Liquid

Product Line

ActinGreen™, ReadyProbes™

Technique

Fluorescence Intensity

Label Type

Fluorescent Dye

Product Type

Phalloidin

SubCellular Localization

Cytoskeleton

For Research Use Only. Not for use in diagnostic procedures.