R&D Systems™ Recombinant Human EGF Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.

Accession Number

P01133

For Use With (Application)

Bioactivity

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS.

Gene ID (Entrez)

1950

Molecular Weight (g/mol)

M.W. (Observed): 6 kDa, reducing conditions; M.W. (theoretical): 6 kDa

Name

EGF

Quantity

200 μg

Source

E. coli-derived human EGF protein Asn971-Arg1023, with an N-terminal Met

Storage Requirements

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.

Endotoxin Concentration

<0.10 EU / 1 μg of the protein by the LAL method.

Gene Symbol

EGF

Conjugate

Unconjugated

Recombinant

Recombinant

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.

Purity or Quality Grade

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.

Protein

EGF