R&D Systems™ Recombinant Human IL-6/IL-6R alpha Protein Chimera, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human IL-6/IL-6R alpha Protein Chimera is derived from HEK293. The Recombinant Human IL-6/IL-6R alpha Protein Chimera has been validated for the following applications: Bioactivity.

For Use With (Application)

Bioactivity

Gene ID (Entrez)

3570

Molecular Weight (g/mol)

Observed M.W.: 78 - 93kDa, reducing conditions, Theoretical M.W.: 59kDa

Name

IL-6R alpha

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining

Quantity

25μg

Source

Human embryonic kidney cell,HEK293-derived human IL-6/IL-6R alpha Complex protein, (N-terminus) Human IL-6R alpha (Leu20-Asp358) Accession &Num; P08887 GGGSGGGSGGGS Human IL-6 Val30-Met212 Accession &Num; P05231 (C-terminus)

Storage Requirements

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.

Endotoxin Concentration

<0.10 EU per 1μg of the protein by the LAL method.

Gene Alias

BSF-2, CD126, gp80, IL6, IL-6 R alpha, IL6Q, IL6R, IL6R alpha, IL-6R-1, IL6RA, IL-6RA, IL6RQ, interleukin 6 receptor, MGI-2A

Common Name

IL-6 R alpha

Gene Symbol

IL6

Biological Activity

Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is typically 2.5-12.5ng/mL.

Conjugate

Unconjugated

Host Species

Human

Recombinant

Yes

Expression System

Human embryonic kidney cell, HEK293-derived Human IL-6 R alpha (Leu20-Asp358) Accession # P08887, N-terminus; GGGSGGGSGGGS; Human IL-6 Val30-Met212 Accession # P05231, C-terminus