R&D Systems™ Recombinant Human VEGF 165 Protein

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.50-12.0 ng/mL.

Accession Number

NP_001165097.1

For Use With (Application)

Bioactivity

Formulation

Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.

Gene ID (Entrez)

7422

Molecular Weight (g/mol)

M.W. Observed: 19-21 kDa, under reducing conditions., M.W. Theoretical: 19.2 kDa (monomer)

Quantity

50 μg

Source

E. coli-derived human VEGF protein Ala27-Arg191

Storage Requirements

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Endotoxin Concentration

<0.01 EU per 1 μg of the protein by the LAL method.

Gene Alias

MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor

Conjugate

Unconjugated

Reconstitution

Reconstitute the 10 μg size at 100 μg/mL in sterile deionized water. Reconstitute all other sizes at 500 μg/mL in sterile deionized water.

Purity or Quality Grade

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Protein

VEGF