R&D Systems™ Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera is immobilized at 2 μg/mL (100 μL/well), Recombinant Mouse RGM-B (Catalog # 3597-RG ) binds with an ED50 of 0.08-0.48 μg/mL. Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera (Catalog #10021-PL) weakly binds to Recombinant Mouse PD-1.

Specifications

• Accession Number: Q9WUL5
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 80380
• Molecular Weight (g/mol): M.W. (Observed): 64-84 kDa, reducing conditions; M.W. (theoretical): 49 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein Mouse PD-L2 (Leu20-Arg219) (Lys113Ser) (N-terminus) Accession # Q9WUL5 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Specific Reactivity: PD-L2/B7-DC/PDCD1LG2
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-L2/B7-DC/PDCD1LG2