R&D Systems™ Recombinant P. chrysogenum APS kinase/APSK Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to phosphorylate adenosine 5'-phosphosulfate (APS). The specific activity is >100 pmol/min/μg, as measured under the described conditions, and is subject to known limitations imposed by a factor of coupling rate (8).

Specifications

• Accession Number: Q12657
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 29-30 kDa, reducing conditions; M.W. (theoretical): 25 kDa
• Name: APS Kinase/APSK
• Quantity: 20 μg
• Source: E. coli-derived p. chrysogenum APS Kinase/APSK protein Met1-Glu211, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane)
• Protein: APS Kinase/APSK