Thermo Scientific™ pTK-Red Firefly Luc Vector for Luciferase Assays

Description

Drive red-shifted firefly luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

The Thermo Scientific™ pMCS-Red Firefly Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the intracellular red firefly luciferase reporter.

The Red Firefly Luc Vectors contain a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

• Intracellular Red Firefly Luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
• Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Specifications

• Description: pTK-Red Firefly Luc Vector
• Type: Luc Vector
• Cloning Method: Restriction Enzyme ⁄ MCS
• Delivery Type: Transfection
• Quantity: 10 μg
• Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
• Reporter Gene: Red Firefly Luciferase
• Selection Agent (Eukaryotic): Puromycin
• For Use With (Application): Reporter Assays
• Promoter: TK

For Research Use Only. Not for use in diagnostic procedures.