Gibco™ RevitaCell™ Supplement (100X)

RevitaCell™ Supplement (100X)

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

Enabling—provides maximum cell viability when performing single cell passaging

Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons

Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence

Single cell passaging
Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.

Higher specificity
Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).

Quicker post-thaw recovery
PSCs recovered at 40,000 viable cells/cm² using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.

Content And Storage

Store at -5 to -20°C and protect from light.

Cell Type

Stem Cells (iPS - Induced Pluripotent Stem)

Classification

Animal Origin-Free

Concentration

100 X

Culture Type

Stem Cell (Embryonic, iPS - Induced Pluripotent Stem)

Product Type

Media Supplement

Manufacturing Quality

cGMP for medical devices, 21 CFR Part 820 and ISO 13485

Product Line

Gibco™, RevitaCell™

Shipping Condition

Dry Ice

Source

Animal origin-free

For Research Use Only. Not for use in diagnostic procedures.