Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

RiboLock RNase Inhibitor inhibits the activity of RNases A , B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

• Does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H
• Performs under a wide range of reaction conditions
• Protects RNA from degradation at temperatures up to 55°C

Quality Control:

• The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases and ribonucleases confirmed by appropriate quality tests
• Functionally tested in RNA and cDNA synthesis

Source:

• E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor

Molecular Weight:

• 49.6kDa monomer Definition of Activity Unit:
  • One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%
  • Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH 7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [³H]-RNA, 50mg/mL yeast RNA, 8mM DTT
  Storage Buffer:
  • The enzyme is supplied in: 20mM HEPES-NaOH (pH 7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol
  Inhibition and Inactivation
  • Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound
  • Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min. heating at 70°

  Recommended for:

  • Inhibition of RNA degradation in the following: in vitrotranscription (1), cDNA synthesis (2), in vitrotranslation (3), isolation of mammalian cell fractions that contain mRNA-protein complex
  • RNA purification and storage
  • Separation and identification of specific ribonuclease activities
  • Studies of tumor suppression

  Note:

  • DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity
  • Recommended concentration 1u/μl of a reaction mixture

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Concentration

40 U/μL

Compatible Buffer

Storage Buffer

Quantity

2500 U

Product Type

RNase Inhibitor

Product Line

RiboLock™

For Research Use Only. Not for use in diagnostic procedures.