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Thermo Scientific Chemicals Safranine O

Catalog No. AAB2167409
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It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Chemical Identifiers

CAS

477-73-6

Molecular Formula

C20H19ClN4

Molecular Weight (g/mol)

350.85

MDL Number

MFCD00011759

InChI Key

QRYAEWIQIBAZOJ-UHFFFAOYSA-N

Synonym

basic red 2, safranine o, gossypimine, safranin, safranine t, safranin o, safranin t, safranine, tolusafranine, hidaco safranine

PubChem CID

2723800

IUPAC Name

3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride

SMILES

[Cl-].CC1=C(N)C=C2C(=C1)N=C1C(C)=C(N)C=CC1=[N+]2C1=CC=CC=C1

Specifications

Odor

Odorless

Quantity

10 g

Beilstein

3924099

Solubility Information

Soluble in water at 20°C,50g/L. Soluble in alcohol.

Formula Weight

350.85

Chemical Name or Material

Safranine O

Hazard Category

H315-H318

Hazard Statement

GHS H Statement
H315-H319-H335
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

Precautionary Statement

P264b-P280-P302+P352-P305+P351+P338-P310-P332+P313-P362

EINECSNumber

207-518-8

RTECSNumber

SG1623000

TSCA

Yes

Recommended Storage

Ambient temperatures

RUO – Research Use Only

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