Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Established medium for desalting and buffer exchange

• Gel filtration medium
• Available in coarse, medium, fine and superfine grades
• All grades satisfy process-scale chromatography requirements
• Available in lab packs or prepacked columns

Specifications:

• Working pH range: 2 to 13
• Bed volume (in distilled water): 4 to 6mL/g
• Fractionation range dextrans/globular proteins: 1× 10² to 5 × 10³ / 1 × 10³ to 5 × 10³ M_r
• Max. flowrate/operating pressure: the beads behave as rigid spheres obeying Darcy's Law

Quantity

500 g

Flow Rate

>47 cm/hr.

Particle Size

17 to 132 μm (Wet Form), >20 μm (Dry Form)

Bed Volume

4 to 6mL/g in Distilled Water

Product Type

Sephadex G-25 Media

Format

Solid Spherical Bead

Diameter (Metric) Inner

2.6cm

For Use With (Application)

Desalting and Buffer exchange and removal free label in industrial applications

Height (English)

11.81 in.

Height (Metric)

30cm

Max. Pressure

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

pH Range

2 to 13

Support Type

Cross-linked Dextran

Content And Storage

4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH