Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Its larger bead size is well-suited for large scale separations, where high flow rates and low operating pressures are required.

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step
• Suitable for purification of DNA from small molecules by gel filtration
• Larger bead size for large scale group separations
• Classic gel filtration medium

Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five G-types, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine).

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Quantity

500g

Flow Rate

< Darcy´s Law

Product Type

Sephadex G50 Media

For Use With (Application)

For the separation of molecules M_r >30 000 from molecules M_r <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids

Max. Pressure

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

pH Range

2 to 10

Content And Storage

4 to 30°C