Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

• Prepared for use in polar organic solvents and aqueous solvent mixtures
• Made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network
• Hydrophilic and lipophilic dual nature of the matrix enables powerful chromatographic selectivity
• Batch-to-batch reproducibility
• Partical size dry: 18μ-111μm
• Fractionation range globular proteins: 4x10³ to 5 x 10³

• Stable in most aqueous and organic eluent systems
• Shelf life: 5years
• Autoclavable: 20 min at 121°C
• Flow Velocity <∽12cm/min
• pH stability working range: 2-13

Analytical and industrial scales for the preparation of closely related molecular species

Quantity

25 g

Flow Rate

720 cm/hr.

Particle Size

18 to 111 μm in dry form

Product Type

Sephadex LH-20 Media

Format

Solid Spherical Bead

Diameter (Metric) Inner

2.6cm

For Use With (Application)

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Height (English)

11.81 in.

Height (Metric)

30cm

Max. Pressure

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

pH Range

2 to 13

Support Type

Hydroxypropylated, cross-linked Dextran

Sample Type

Steroid, Terpenoid, Lipid, low molecular weight Peptide

Content And Storage

4 to 30°C