Cytiva Sephaorose™ Fast Flow Anion Exchange Media: DEAE

Used for preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ Sephaorose™ Fast Flow Anion Exchange Media: DEAE ensures low levels of non-specific binding.

Supplier:  Cytiva 17070901

Catalog No. 45-002-929

Description

• Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
• Matrix is composed of crosslinked 6% agarose beads
• Part of Sepharose Fast Flow ion exchange platform
• High chemical stability allows well proven cleaning-in-place (CIP) and sanitization protocols
• Available in range of pre-packed process development tools

• Ligand: Diethylaminoethyl
• Ion exchanger type: weak anion exchanger
• Ionic capacity: 0.11 to 0.16mmol Cl^-/mL medium
• Binding capacity: 110mg HSA/mL medium
• Pressure/Flow specification: 300 to 600cm/hr., 100kPa, XK 50/30 column, bed height 15cm

Capture and purification of proteins.

Specifications

• Quantity: 500 mL
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
• Product Type: DEAE Sepharose Fast Flow Media
• Particle Size: 45 to 165 μm
• Height (Metric) Bed: 15 cm
• Ion Exchange Type: Strong AnIon Exchanger
• Matrix: 6% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol

• For Use With (Application): For Preparative Protein Separations in Both Research and Industrial AppliCatIons
• Storage Requirements: 4°C to 30°C
• Flow Rate: 400 to 700 cm/Hoμr
• Height (English) Bed: 5.9 in.
• Ionic Capacity: 0.11 to 0.16 mM Cl-/mL
• Max. Pressure: 100 kPa
• pH Range: 2 to 14
• Ligand Type: Diethylaminoethyl