Thermo Scientific™ Slide-A-Lyzer™ Dialysis Flasks

Facilitate removal of salts and small contaminants from proteins or macromolecules in sample volumes up to 250 mL. Each Slide-A-Lyzer Dialysis Flask provides flotation disks that when attached to the neck of the flask, allows the flask to float in a vertical position for simple and safe handling.

Slide-A-Lyzer Dialysis Flasks (2K, 3.5K, 10K, or 20K MWCO) facilitate simple and effective removal of buffer salts and small contaminants in sample volumes up to 250 mL. They are easy to use: add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity.

The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: purple (20,000 Daltons), orange (10,000 Daltons), pink (3,500 Daltons), and blue (2,000 Daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

Features of Slide-A-Lyzer Dialysis Flasks:
• Easy to use—simply pipette or pour sample into flask and begin dialysis
• Fast dialysis—flat flask chamber with two membranes provides high surface area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
• High recovery—rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
• Multiple molecular-weight cut offs—select the membrane MWCO that best suits your sample's molecular weight

Dialysis membrane specifications:
• Composition: regenerated-cellulose
• Thickness: 0.9–1.2 mil (22.5 to 30 μm); 2K MWCO = 2 mil (50 μm)
• Hydration time required before use: 2 minutes
• Glycerol content: trace (10K MWCO content is 21%)
• Sulfur content: 0.04–0.17%
• Heavy metals content: trace

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange, and desalting can be accomplished in eight hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation, and temperature. Minimum dialysis time must be determined empirically for each specific system.

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Description

Dialysis Flasks, 10K MWCO

Content And Storage

Shipped at ambient temperature. Upon receipt, store at room temperature.

Purification Target

Buffer Exchange, Protein

Membrane

Cellulose Membrane, Dialysis

Format

Flask

MWCO

10 kDa

Product Line

Slide-A-Lyzer™

Quantity

4 Flasks

Sample Volume (Metric)

250 mL