Thermo Scientific™ SnakeSkin™ Dialysis Tubing

Perform desalting and buffer exchange for 10 to 100 mL samples with this 3.5K, 7K, and 10K molecular weight cut-off (MWCO), ready-to-use form of traditional dialysis membrane tubing. Securely close dialysis tubing up to 50 mm flat diameter with the sturdy, non-floating tube SnakeSkin Dialysis Clips.

The SnakeSkin 3.5K, 7K, and 10K MWCO dialysis tubing is an easy and ready-to-use traditional dialysis membrane tubing that allows desalting and buffer exchange for samples of 10 to 100 mL. SnakeSkin Dialysis Tubing Clips are sturdy, non-floating tube closures recommended for use with SnakeSkin Dialysis Tubing, but are suitable for any dialysis tubing with flat tube diameters up to 50 mm.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, then add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, so it matches the dialysis performance of conventional tubing.

Features of SnakeSkin Dialysis Tubing:
• Convenient—ready to use, pre-wetted, pleated tube
• High recovery—greater than 90% protein recovery
• Speed—dialysis is generally completed in 4–6 hours
• Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents, and alcohols

Features of SnakeSkin Dialysis Tubing Clips:
• Durable—polyamide composition for long-life
• Dove-tail latch—prevent sample loss
• Heat resistant—autoclave up to 134°C

SnakeSkin Dialysis Tubing, 3.5K or 10K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. It is supplied in 20-cm sticks containing 35 feet of dialysis tubing having a 16-, 22-, or 35-mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23–50 mm diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ∼2–10 mL of sample per centimeter of length.

SnakeSkin 7K MWCO dialysis tubing is supplied in 20-cm sticks containing 35 feet of dialysis tubing having a 22-mm circular internal diameter (I.D.). Equivalent in capacity to 10.5 meters of 34-mm diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ∼3.7 mL of sample per centimeter of length.

3.5K MWCO tubing specifications:
• Membrane type: regenerated cellulose
• Tubing nominal dry thickness: 0.9–1.2 mil (22–30 μm)
• Glycerol content: trace
• Sulfur content: 0.1–0.15%
• Heavy metals content: trace

7K MWCO tubing specifications:
• Membrane type: regenerated cellulose
• Tubing nominal dry thickness: 0.9–1.2 mil (22–30 μm)
• Glycerol content: 13%
• Sulfur content: 0.1–0.15%
• Heavy metals content: trace

10K MWCO tubing specifications:
• Membrane type: regenerated cellulose
• Tubing nominal dry thickness: 0.9–1.2 mil (22–30 μm)
• Glycerol content: 21%
• Sulfur content: 0.1–0.15%
• Heavy metals content: trace

Dialysis clip specifications:
• Height: 10 mm
• Width: 16 mm
• Inner Length: 50 mm (maximum closure width)
• Outer Length: 66 mm (end to end)
• Composition: polyamide (nylon) derivative

Product Type

Tubing

Product Line

SnakeSkin™

MWCO

10 kDa

Membrane

Cellulose Membrane, Dialysis

Material

Cellulose

Format

Tubing

Diameter (Metric)

35 mm I.D.

Description

Dialysis Tubing, 10K MWCO, 35 mm I.D.

Purification Target

Buffer Exchange, Protein

Sufficient For

Approx. 48 mL per 5 cm length (plus 2.5 cm at each end for closure) yields 100 uses

Quantity

10.5 m

Content And Storage

Upon receipt store at room temperature or 4°C. Keep in closed original packaging tube to prevent desiccation, which results in cracks or pinholes in the membrane. For best results, keep membrane hydrated by occasionally adding 1-2 drops of distilled water to the packaging tube.