Sodium/Calcium Exchanger Monoclonal Antibody (C2C12), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA3926

Catalog No. MA3926

Description

MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues. MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane. The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.

Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types. NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NaCX (NaCX-1, NaCX-2, and NaCX-3), and two in the NaCKX family (NaCKX-1 and NaCKX-2). NaCX-1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling (Matsuoka et al., 1993). NACX-1 may also be a target for protection of the heart and brain from ischemia and reperfusion injury (Hagihara et al., 2005; Bano et al., 2005; Choi, 2005).

Specifications

• Antigen: Sodium/Calcium Exchanger
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P23685, P32418, P48766, P70414, Q01728
• Gene Symbols: SLC8A1
• Immunogen: Purified canine cardiac sodium/calcium exchanger.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Canine, Guinea Pig, Human, Mouse, Rabbit, Rat
• Product Type: Antibody
• Isotype: IgM

• Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: C2C12
• Conjugate: Unconjugated
• Gene: SLC8A1
• Gene Alias: AI852629; AV344025; cardiac sarcolemmal sodium-calcium exchanger; CNC; D930008O12Rik; isoform NCX1.13; isoform NCX1.3; isoform NCX1.9; Na(+)/Ca(2+)-exchange protein 1; Na/Ca exchanger; Na/Ca exchanger NACA1 isoform; Na+/C2+ exchanger; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; NACA; Na-Ca exchanger; Ncx; Ncx1; NKX; renal Na/Ca exchanger NACA-2; sarcolemmal sodium/calcium exchanger; SLC8A1; sodium/calcium exchanger; sodium/calcium exchanger 1; sodium/calcium exchanger isoform NaCa13; sodium/calcium exchanger isoform NaCa3; sodium/calcium exchanger isoform NaCa7; sodium/calcium exchanger isoform NaCa9; sodium-calcium exchanger; solute carrier family 8 (sodium/calcium exchanger), member 1; solute carrier family 8 member 1; solute carrier family 8 member A1; solute carrier family 8, member 1
• Host Species: Mouse
• Purification Method: Affinity chromatography - MBP
• Regulatory Status: RUO
• Gene ID (Entrez): 100135620, 100328570, 20541, 29715, 475738, 6546
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid