MilliporeSigma™ Stem Cell Qualified ECM Gel

Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture.

• Stem Cell Qualified ECM Gel is a soluble basement membrane extract (BME) purified from Engelbreth-Holm-Swarm (EHS) tumor that has been pre-qualified for human ES/iPS cell culture
• The matrix polymerizes at 20 to 40°C to form a reconstituted basement membrane
• ECM Gel contains laminin, collagen IV, entactin and heparan sulfate among other ECMs
• The matrix has been extensively tested and optimized for human ES/iPS cell culture
• Eliminates the need for time-consuming prescreening and lot identification
• Provides an optimized ECM coating necessary for long term culture of pluripotent human ES/iPS cells
• Supports feeder-free culture of human ES/iPS cells in serum-free media
• Compatible with all feeder free human ES/iPS cell expansion media (i.e. mTeSR™, PluriSTEM™)

Assays

< 5% spontaneous differentiation for 3 passages using Human iPS cells

Color

Red, clear of particulates

Content And Storage

Store at -20°C, thaw overnight on ice in a 2 to 8°C refrigerator

Description

Basement membrane extract (BME)

Form

Gel

Endotoxin Level

< 20 EU/mL

For Use With (Application)

Human ES and iPS cell culture

Quantity

5 mL