Thermo Scientific™ Super Heavy TMTpro™ Label Reagent

The amine-reactive Super Heavy TMTpro Label Reagent is an alternate form of TMTpro that contains twice the number of heavy isotopes for labeling peptides. When used with TMTpro-labeled samples, it enables 2D TMTpro multiplexing applications for targeted quantitation by mass spectrometry.

Super Heavy TMTpro Label Reagent labels synthetic peptides to be specifically used alongside TMTpro multiplex-labeled samples for targeted multiplexed quantitation by mass spectrometry analysis.

Super Heavy TMTpro Label Reagent can:
• Provide two-dimensional multiplexing—super heavy-labeled TMTpro peptides used alongside TMTpro-labeled samples allows multiplexed targeted quantitation with mass spectrometry analysis
• Improve quantitative accuracy—label reduces co-isolation and interference to enable robust quantitation
• Reduces acquisition complexity—heavy-labeled peptide used as the trigger for peptide selection

When used in combination with TMTpro labeled samples, the mass offset from the super heavy-labeled synthetic peptide serves as a trigger for the selection of the targeted peptide in the multiplexed sample. This triggered selection reduces interference/co-isolation to enable more robust quantitation using mass spectrometry analysis. This new method combines the power of multiplexing from TMTpro reagents with targeted quantitation methods and is referred to as the TOMAHAQ (Triggered by Offset, Multiplexed, Accurate mass, High resolution, and Absolute Quantitation) method. An additional advantage of the TOMAHAQ method for targeted multiplexed quantitation is less dependence on the chromatography conditions for successful quantitation of peptides.

This product is subject to a limited use label license.

Product Type

Label Reagent

Product Line

TMT™

Synonym

TMTpro

Starting Material

Peptides

Final Product Type

Peptides

Detection Method

Mass Spectrometry

Workflow Step

Peptide labeling

Quantity

2 mg

Color

White

Packaging

Plastic Vial

Purity

93%

Grade

MS

Molecular Formula

C4[13 C]15H30N[15 N]3O6

Form

Solid