Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladder

Ready-to-use mix of recombinant proteins (20 to 150K) with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection methods.

Thermo Scientific™ SuperSignal Molecular Weight Protein Ladders (20 to 150KDa) are a ready-to-use mix of recombinant proteins with IgG-binding sites that provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems.

The SuperSignal Molecular Weight Protein Ladders and SuperSignal Enhanced Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The molecular weight marker mix also contains a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods.

Two versions of the standard are offered. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. The enhanced standard is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used.

Highlights:

• Ready-to-use – liquid format is ready to load and requires no boiling

• Direct visualization – see marker bands with gel stains and blotting detection reagents

• Consistent – even band intensity regardless of detection method

• Compatible – enhanced version for compatibility with mouse monoclonal primary antibodies

• Simple marker sizes – band molecular weights are divisible by 10K, making them easy to remember

• Stable – store long term at -20°C, three months at 4°C or one month at ambient temperature

Additional Features:

• The molecular masses included in these standards are 20, 30, 40, 50, 60, 80, 100 and 150kDa.

Content And Storage

Contents: one vial of 250 μL

Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol

Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient

Detection Method

User defined detection system

Number of Markers

8

Product Type

Protein Ladder

Ready to Load

Yes

Size Range

20 to 150 kDa

Stain Type

Unstained

Gel Compatibility

Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

Molecular Weight (g/mol)

150, 100, 80, 60, 50, 40, 30, 20 kDa

Product Line

SuperSignal™

Quantity

250 μL

Shipping Condition

Wet Ice

System Type

Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.